International Journal of Chemical Studies
Vol. 6, Issue 2 (2018)
Molecular detection of citrus yellow mosaic virus (CYMV) and citrus greening bacterium (CGB) in sweet orange by multiplex polymerase chain reaction
Author(s): KN Gupta and VK Baranwal
Abstract: Citrus is one of the most economically important fruit crop in India. Commercially grown citrus includes sweet orange, acid lime and mandarin. Citrus Yellow Mosaic (CYMV), a viral disease and citrus greening bacterium (CGB), a bacterial disease are the two most important diseases that are impending fruit production through the world. A method of multiplex polymerase chain reaction (PCR) was developed for the simultaneous detection of Citrus yellow mosaic virus (CYMV) and citrus greening bacterium, Candidatus Liberibacter asiaticus (CLa) from sweet orange trees. Initially total DNA from individual CLa and CMBV infected citrus plants were mixed infected field sample for both pathogens were detected by Multiplex PCR. Using multiplex PCR two different fragments of 1024 bp and 451 bp specific to CYMV and CGB respectively were simultaneously amplified. The consistent result of multiplex PCR was compared with Simplex PCR for detection of each pathogen. The Muliplex PCR method developed in the present investigation proved to be highly sensitive, economic and reliable methods for detection of citrus greening bacterium (CGB) and the Citrus yellow mosaic virus (CYMV) in citrus trees from the orchards. The technique should prove highly useful in disease surveys, nursery certification and quarantine applications.
Pages: 888-891 | 383 Views 4 Downloads
How to cite this article:
KN Gupta, VK Baranwal. Molecular detection of citrus yellow mosaic virus (CYMV) and citrus greening bacterium (CGB) in sweet orange by multiplex polymerase chain reaction. Int J Chem Stud 2018;6(2):888-891.