International Journal of Chemical Studies
Vol. 6, Issue 1 (2018)
In vitro somatic embryogenesis and regeneration from epicotyl segments of Rough lemon (Citrus jambhiri Lush.)
Author(s): Sukhjit Kaur
Abstract: Rough lemon (Citrus jambhiri Lush.) is an important rootstock for a number of citrus fruit crops including lemons, oranges, grape fruits, kinnows and mandarins. The objective of this study was to develop an efficient protocol for in vitro embryogenic callus induction and regeneration of rough lemon (Citrus jambhiri Lush.). Epicotyl segments excised from in vitro raised seedlings were used as explants. Different growth regulators media were tried to produce somatic embryoes from epicotyls segments of rough lemon. Only MS medium supplemented with 2,4-D1.0mg/litre + BAP1.0 mg/litre produces early and highest percentage of callus with formation of somatic embryoes. Among different somatic callus regeneration media, MS medium fortified with NAA0.5mg/litre, BAP3.0 mg/litre and kinetin1.0mg/litre having good regeneration potential with minimum number of days taken for regeneration, highest number of shoots and shoot length. Rooting of regenerated shoots was highest in MS supplemented with NAA1.0mg/litre and IBA1.0 mg/litre- with minimum number of days taken to rooting, highest roots and root length. The plantlets were successfully acclimatized in different potting mixtures and highest survival rate (96.45%) was achieved in potting mixture containing garden soil+ sand+ vermiculite (1:1:1).
Pages: 2082-2091 | 405 Views 4 Downloads
How to cite this article:
Sukhjit Kaur. In vitro somatic embryogenesis and regeneration from epicotyl segments of Rough lemon (Citrus jambhiri Lush.). Int J Chem Stud 2018;6(1):2082-2091.