International Journal of Chemical Studies
Vol. 6, Issue 1 (2018)
Method validation for analysis of pesticide residues in Labeo rohita fish through GC-MS/MS
Author(s): Rajesh Kumar Sahu and Krishnaiah Nelapati
Abstract: The present study was undertaken to develop and validate multiresidue method (MRM) for twenty four pesticide residues in Labeo rohita fish using Gas Chromatography Triple Quadrupole Mass Spectrometer (GC-MS/MS). The method was based on QuEchERs method (quick, easy, cheap, efficient, rugged and safe) extraction with acetonitrile and dispersive solid phase extraction (d-SPE) cleanup with PSA and MgSO4. At spiking level 0.01, 0.05 and 0.1 µg mL-1 recovery studies were conducted and percent recovery found to be within acceptable limit except synthetic pyrethrnoids at spiking level 0.01 µg mL-1. All RSD values were below 20%. The coefficient of determination (R2) for all pesticide ≥ 0.992. The developed method was validated and found to be fast, simple and reliable for analysis of pesticide residues in fish samples.
Pages: 1448-1452 | 392 Views 22 Downloads
How to cite this article:
Rajesh Kumar Sahu, Krishnaiah Nelapati. Method validation for analysis of pesticide residues in Labeo rohita fish through GC-MS/MS. Int J Chem Stud 2018;6(1):1448-1452.