International Journal of Chemical Studies
Vol. 6, Issue 1 (2018)
Effect of Cysteamine, Epidermal Growth Factor (EGF) and Estrous Cow Serum (ECS) on In-vitro Maturation of Bovine Oocytes
Author(s): M Bhajoni, D Bhuyan, RK Biswas, DJ Dutta, H Dev and G Hazarika
Abstract: A total of 526 good quality bovine oocytes were subjected to in-vitro maturation of oocytes at 38.5 0C in humidified atmosphere of 5% CO2 tension for 24 hours and matured in-vitro in medium-I or control (TCM-199+10% FBS+L-glutamine+sodium pyruvate+Gentamicin+pFSH+ Estradiol 17-β), medium-II (control+5% ECS), medium-III (control+100µM/ml cysteamine), medium-IV (control+10ng/ml EGF) and medium-V (control+ 5% ECS+ 100µM/ml cysteamine+10ng/ml EGF). The rates of maturation based on cumulus cell expansion and nuclear maturation varied significantly (p<0.01) between media being the highest in the medium-IV (86.92% and 62.36%) containing epidermal growth factor (EGF) followed by medium-III (82.24% and 56.82%) containing cysteamine among all the media.
Pages: 1444-1447 | 404 Views 9 Downloads
How to cite this article:
M Bhajoni, D Bhuyan, RK Biswas, DJ Dutta, H Dev, G Hazarika. Effect of Cysteamine, Epidermal Growth Factor (EGF) and Estrous Cow Serum (ECS) on In-vitro Maturation of Bovine Oocytes. Int J Chem Stud 2018;6(1):1444-1447.