International Journal of Chemical Studies
Vol. 6, Issue 1 (2018)
Decellularization of bubaline gallbladder using 0.5% Sodium dodecyl sulfate and seeding of stem cells over decellularized matrices
Author(s): Ajit Kumar Singh, Naveen Kumar, SK Maiti, Sameer Srivastava, KP Singh, PDS Raghuwansi and B Om Preethi
Abstract: Decellularization is a process which removes of all the cellular and nuclear material while minimizing any adverse effect on the composition, biological activity, and mechanical integrity of the remaining extra cellular matrix. Various methods of decellularization are used to decellularize the tissues like specific physical, chemical, and enzymatic methods. Sodium dodecyl sulfate (SDS) is an ionic biological detergent with a long-standing record in tissue engineering for decellularization of tissues. Stem cells are the pluripotent cells, capable of differentiation into numerous cells types. Decellularized extracellular matrices provide a native framework for cell adhesion at the site of a tissue deficit, therefore allowing local cells to migrate into the matrix, adhere and undergo differentiation. Thus prepared seeded matrices have wide range of uses in regenerative medicine. In the present study, we developed a biomaterial by decellularizing the bubaline gallbladder with 0.5% SDS and seeding the stem cells over it.
Pages: 962-965 | 532 Views 13 Downloads
How to cite this article:
Ajit Kumar Singh, Naveen Kumar, SK Maiti, Sameer Srivastava, KP Singh, PDS Raghuwansi, B Om Preethi. Decellularization of bubaline gallbladder using 0.5% Sodium dodecyl sulfate and seeding of stem cells over decellularized matrices. Int J Chem Stud 2018;6(1):962-965.