Vol. 6, Issue 1 (2018)
Biosynthesis characterization and antibacterial activity of bioiron nanoparticles synthesized by Pseudomonas putida strain LUA 15.1
Author(s): Neha Sharma, Himani Sharma, Praveen Khatri and Poonam Shirkot
Abstract: Bioiron nanoparticles have been used as antimicrobial agents, bioremediation of waste material, effluent detoxification, decolorization of dyes and pesticides degradation. In present investigation, in vitro synthesis of bioiron nanoparticles by Pseudomonas putida LUA 15.1 was carried out by using various optimization parameters. Optimum condition parameters for maximum synthesis of bioiron nanoparticles was selected which included nutrient broth containing 5g/l peptone, 3g/l beef extract, 3g/l NaCl and 2.0mM FeSO4. Nutrient broth (pH; 8.0) using optimum condition was used for in vitro synthesis of bioiron nanoparticles at 35°C for 24 hrs of incubation. Nanoparticles synthesis indicated by change in colour from pale yellow to orange was confirmed by UV–Vis spectroscopy with absorbance peak obtained between 300-550 nm. Characterization of bioiron nanoparticles was carried using FTIR, XRD DLS and SEM. These bioiron nanoparticles found to be monodispersed as well as in aggregates with triangular/square/rectangular/rod shaped with size range of 75nm and FTIR spectrum revealed presence of alchol-phenol, alkenes, aromatic, alkane, alkyl halide, amine, and ether biomolecules and these were tested for antibacterial activity against two bacterial plant using agar well diffusion test producing significant zones of inhibition against bacterial pathogens after 24 hrs of incubation.
Pages: 691-699 | 696 Views 43 Downloads
How to cite this article:
Neha Sharma, Himani Sharma, Praveen Khatri, Poonam Shirkot. Biosynthesis characterization and antibacterial activity of bioiron nanoparticles synthesized by Pseudomonas putida strain LUA 15.1. Int J Chem Stud 2018;6(1):691-699.