International Journal of Chemical Studies
Vol. 6, Issue 1 (2018)
Effect of plant growth regulators and sucrose concentration on callus induction and shoot differentiation from ovary culture of marigold (Tagetes spp).
Author(s): Thaneshwari, C Aswath
Abstract: Marigold (Tagetes spp) an important ornamental plant, is also recognized as potential source of carotenoids and currently being used as nutritional supplements, food colorants and poultry feed additives. Most of the hybrids and varieties of marigold have been developed by conventional method of breeding. In vitro androgenesis and gynogenesis is a rapid and efficient method for developing new varieties in a relatively short time. The present paper reports the effect of growth regulators and the level of sucrose on callus induction from ovary culture of marigold cultivar 'Arka Agni'. Ovaries were cultured on a MS medium supplemented with seventeen different combinations of plant growth regulators. Seven levels of sucrose were evaluated. Variable callogenic responses were expressed on induction media supplemented with varying concentration of growth hormones. Pale green colour compact calli with 89.66% callus induction rate has been reported in MS media supplemented with 4.44 µM BAP and 4.52 µM 2,4-D. 10.08% shoot differentiation rate has been reported when calluses were sub cultured on MS media supplemented with 4.44 μM BAP and 1.07 μM NAA. MS media without plant growth regulator was found to be the best rooting medium. 4% sucrose concentration on MS media was found to be optimum for callus induction. In summary, the present study shows positive result in terms of regenerating marigold plantlets from ovary culture. There is a need for testing of the plantlets produced in terms of genetic variation as well as the ploidy level. Since there is a high genotypic dependence of the ability for gynogenesis, more studies involving different marigold cultivars are required.
Pages: 618-623 | 1953 Views 22 Downloads
How to cite this article:
Thaneshwari, C Aswath. Effect of plant growth regulators and sucrose concentration on callus induction and shoot differentiation from ovary culture of marigold (Tagetes spp).. Int J Chem Stud 2018;6(1):618-623.