International Journal of Chemical Studies
Vol. 6, Issue 1 (2018)
Analysis of purified lycopene extract obtained from fresh tomatoes
Author(s): Ruby Siwach and Jayanti Tokas
Abstract: Lycopene is a carotenoid, widely known for its antioxidant properties. In present investigation an attempt was made to observe the effects of purification process on antioxidant activity and isomerisation of lycopene. Antioxidant activity of crude as well as purified lycopene extract was measured as Trolox Equivalent Antioxidant Capacity (TEAC) using ABTS (2,2-azinobis-3-ethylbenzothiazoline-6-sulfonate) method. Crude as well as purified lycopene extract were subjected to spectrophotometric analysis for identification of peaks. HPLC analysis of crude as well as purified lycopene extract was carried out for cis – trans identification of peaks. Purification process significantly (p<0.05) increased the antioxidant activity of crude lycopene extract. Analysis of HPLC chromatograms revealed that isomerisation of lycopene did not take place during purification process and no cis-isomers were observed in purified lycopene.
Pages: 597-601 | 339 Views 6 Downloads
How to cite this article:
Ruby Siwach, Jayanti Tokas. Analysis of purified lycopene extract obtained from fresh tomatoes. Int J Chem Stud 2018;6(1):597-601.