Vol. 6, Issue 1 (2018)
Evaluation of hydroxycinnamic acids in finger millet (Eleusine coracana) grain cultivars by HPTLC
Author(s): Giridhar Goudar, Sathisha GJ, Mahadev C Khetagoudar, Vasanthraj B and Sameer R Patil
Abstract: Finger millet (Eleucine coracana) grain cultivars were analyzed for the phenolic components (hydroxycinnamic acids) which have beneficial effects on human health. The present study was conducted to evaluate the phenolic acids viz., ferulic acid and caffeic acid in four different varieties of finger millet. Phenolic compounds were extracted in grain samples and were primarily evaluated for total phenolic content (TPC), total flavonoid content (TFC) and antioxidant property. TPC was analyzed by Folin-Ciocalteu method, TFC by aluminium chloride method and antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Further, the sample extracts were subjected to High Performance Thin Layer Chromatography (HPTLC) analysis and the method was standardized for the evaluation of ferulic acid and caffeic acid, identification was done by comparing obtained Rf values of the reference standards with the sample extracts and quantification was done using the peak areas of the obtained chromatograms. Results revealed that TPC (949.57 GAEµg/g) was high in GPU28, whereas TFC (805.16 REµg/g) and antioxidant activity (53.38 I%) was found to be high in GPU48 variety.
Pages: 328-332 | 882 Views 26 Downloads
How to cite this article:
Giridhar Goudar, Sathisha GJ, Mahadev C Khetagoudar, Vasanthraj B, Sameer R Patil. Evaluation of hydroxycinnamic acids in finger millet (Eleusine coracana) grain cultivars by HPTLC. Int J Chem Stud 2018;6(1):328-332.