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P-ISSN: 2349-8528, E-ISSN: 2321-4902   |   Impact Factor: GIF: 0.565 NAAS Rating: 5.31 NEW

International Journal of Chemical Studies

Vol. 5, Issue 6 (2017)
Evaluation of DMAB test for detection of urea mixed in milk and improvement in its efficacy


Author(s): Chauhan Mahipalsinh P, Arpita Agnihotri, AI Shaikh, SI Patel and KD Aparnathi

Abstract: Four different categories (carbohydrates, cheaper fats and vegetable oils, non-protein nitrogenous compounds and salts) are used. Amongst the NPN compounds use of urea is most widely reported. DMAB test is one of the most commonly used qualitative tests for detection of urea in milk. For optimization of qualitative tests, different aspects were selected. Different approaches such as selection of medium (milk itself, whey) to perform the test and modification of DMAB reagent by using methanol instead of ethanol in performing the tests to get better results. Depending on the nature of the adulterant and qualitative test to be performed for its detection, a number of approaches were attempted to bring about the improvement in the test. The outcome of the present study suggested a definite improvement in the performance of DMAB tests for detection of urea adulterant in milk. Some of the notable outcomes are ease in judgement due to better differentiation between results of control and test samples, improved sensitivity due to reduction in limit of detection, lowering risk of health hazards as well as environmental pollution due to elimination of corrosive/toxic/poisonous chemical and/or to overcome difficulties in getting some of the prohibited chemicals due to elimination of their use. Moreover, the LoD of urea in whey was 0.06, whereas, in milk was 0.2.

Pages: 1572-1576  |  411 Views  11 Downloads

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How to cite this article:
Chauhan Mahipalsinh P, Arpita Agnihotri, AI Shaikh, SI Patel, KD Aparnathi. Evaluation of DMAB test for detection of urea mixed in milk and improvement in its efficacy. Int J Chem Stud 2017;5(6):1572-1576.
International Journal of Chemical Studies