Vol. 5, Issue 6 (2017)
Electrolyte balance in functional organs and muscle tissue of Clarias gariepinus injected with ethanolic extracts of Lepidagathis alopecuroides
Author(s): Obomanu FG and Gabriel UU
Abstract: Clarias gariepinus were subjected to intramuscular injection of prepared concentrations (2.00, 4.00, 6.00, 8.00 and 10.00ppm) and a control (0.00 ppm) of ethanolic extracts of Lepidagathis alopecuroides leaves at a dose level of 2 ml/ Kg. The fishes were killed on the fourteenth day and dissected. The organs (gill, kidney, liver and muscle) were excised and the levels of different electrolytes in these organs assessed. The results showed a fluctuation in levels of sodium ion in the gill against the control value, (208.75±5.62 Meq/L), lower than control values in the experimental concentrations except at 8.00 ppm where value was equal to that of the control (240.00±8.03 Meq/L) in the kidney. Sodium values were higher than those of the control in the liver and muscle. The highest level of sodium was observed in the gill at 10.00 ppm which was 512.50±4.58 Meq/L. Potassium ion levels were higher than those of the control values in the gills, but were either higher, equal or lower than those of the control values in the kidney, liver and muscle. Potassium ion was highest in the muscle at 2.00 ppm dose concentration which was 89.13±1.08 Meq/L. Chloride ions values in the gills were either lower or higher than the values obtained in the control (218.75±1.91 Meq/L). There was a general decline in the levels of chloride ion in the kidney when compared to the control value (167.13±9.85 Meq/L) except at 4.00 ppm where the value was 168.75±8.87 Meq/L. Chloride ion in the liver were only higher than the control values at 6.00 ppm (302.50±7.94 Meq/L) and 8.00 ppm (402.50±2.91Meq/L). There was a general rise in the levels of the chloride ions in the muscle in all the test concentrations except at 10.00 ppm concentration. There was a general decrease in the levels of hydrogen carbonate ion in the gill and the kidney when compared to their corresponding control values, but fluctuated against that of the control value in the liver, but higher than control values (18.75±7.22 Meq/L) in the muscle. The results obtained indicated a Lepidagathis alopecuroides based toxicity to Clarias gariepinus which can be studied by looking at each organ or tissue separately.
Pages: 1517-1521 | 492 Views 10 Downloads
How to cite this article:
Obomanu FG, Gabriel UU. Electrolyte balance in functional organs and muscle tissue of Clarias gariepinus injected with ethanolic extracts of Lepidagathis alopecuroides. Int J Chem Stud 2017;5(6):1517-1521.