International Journal of Chemical Studies
Vol. 5, Issue 6 (2017)
Genetic diversity analysis in cowpea (Vigna unguiculata (L.) Walp.) Genotypes for storage seed protein profile
Author(s): Ashvini Koolwal and Kuldeep Jangid
An investigation was carried out on 58 genotypes (accessions and mutants of cowpea variety RC 101 and RC 19) of cowpea for seed storage protein profile using Sodium Dodecyl Sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to study diversity among the genotypes. The storage proteins were extracted from cotyledons of the seeds to represent both water and salt soluble proteins. Gels revealed distinct bands within the molecular weight (MW) range of 80 to 14 kD. The binary data was generated by scoring the presence and absence of every protein bands and used to compute Jaccard’s similarity coefficient with the help of NTSYS-pc programme. The similarity coefficient matrix was used to construct a dendrogram of the 58 cowpea genotypes by the un-weighted paired group method using arithmetic averages (UPGMA).
Pages: 1227-1230 | 487 Views 4 Downloads
How to cite this article:
Ashvini Koolwal, Kuldeep Jangid. Genetic diversity analysis in cowpea (Vigna unguiculata (L.) Walp.) Genotypes for storage seed protein profile. Int J Chem Stud 2017;5(6):1227-1230.