International Journal of Chemical Studies
Vol. 5, Issue 6 (2017)
Comparative studies on isolation, identification and purification of Colletotrichum capsici causing anthracnose disease of chilli
Author(s): Veerendra Gupta, Arvinder Kaur, Pramod K Fatehpuria and Himanshu Shekhar Garg
Abstract: Chilli (Capsicum annuum) is valued for its diverse commercial uses. It is one of the major vegetable crops that are grown throughout the world especially in tropical and subtropical regions. India is a major producer, exporter and consumer of chilli. In India, the major chillies growing states are Andhra Pradesh, Karnataka, Maharashtra, Orissa, Tamil Nadu, Madhya Pradesh and Rajasthan. In Madhya Pradesh chilli is grown under an area of 54.41 thousand ha with production of 93.57 thousand MT and productivity 0.98 ton per ha. Anthracnose disease can occur on leaves, stems, and both pre- and post-harvest fruits. Colletotrichum spp. are among the most important plant pathogen worldwide, causing the economically important disease anthracnose (die back or fruit rot, leaf spot, wilt, damping off, etc) in a wide range of hosts, including cereals, legumes, vegetables and tree fruits. Anthracnose of chilli (Capsicum annuum L.) caused by Colletotrichum capsici (Syd.) Butler and Bisby, is one of the major and devastating diseases of chilli causes severe losses (10-60%) both in yield and quality of the chilli depending upon the varieties. The present investigation was aimed to make comparative studies on Isolation, identification and purification of Colletotrichum capsici causing anthracnose of chilli. The present investigation was conducted at Department of Plant Pathology, college of Agriculture, RVSKVV Gwalior (M.P.) using Completely Randomized Block Design (CRD) during kharif 2015-16. Findings of the present investigation revealed that after purification of Colletotrichum capsici were identified on the basis of colony colour, pattern of growth and pattern of acervuli formation on PDA. The fungus produced fairly white to light mouse grey, circular, fluffy mycelium with black colour acervuli which were scattered all over the colony growth against light with the naked eyes and later confirmed with the help of microscope. Microscopic examination of different isolates revealed that the mycelium was septate bearing aseptate unbranched conidiophores. Conidia were sickle shaped, hyaline, unicellular, and fusiform curved with narrow ends. The average dimensions of conidia which possessed large oil globule in the centre, Acervuli contained abundant dark brown niddle like septate setae with several septations and pointed brown tips. After the pathogenicity test of Colletotrichum capsici (test fungus) the fungus was produced same symptoms on the crop.
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How to cite this article:
Veerendra Gupta, Arvinder Kaur, Pramod K Fatehpuria, Himanshu Shekhar Garg. Comparative studies on isolation, identification and purification of Colletotrichum capsici causing anthracnose disease of chilli. Int J Chem Stud 2017;5(6):744-747.