International Journal of Chemical Studies
Vol. 5, Issue 6 (2017)
Genetic variability among variety of sorghum [Sorghum bicolor (L.) Moench] based on protein profile of seed storage proteins
Author(s): DG Gend, VB Jadhav and SN Kolekar
Abstract: In India, the Government enacted the legislation on Protection of Plant Varieties and Farmers Rights (PVP and FR) Act in 2001. The act provides protection of new varieties including extant-notified and farmer’s varieties. Novelty, Distinctness, Uniformity and Stability are the essential requirements for grant of protection to all the new varieties. Under the PVP and FR Act 2001 of India, it requires registration of varieties based on three principles know as DUS (Distinctness, Uniformity and Stability) criteria. The DUS testing principles are used for the protection of variety and award of Plant Breeder’s Rights (PBR), a system of intellectual property protection which is available to breeders of all types of crops. On practical level, DUS assessment of agricultural crops generally involves growing field crops under appropriate ambient conditions, and recording various morphological characteristics of the seed and/or growing plants. Laboratory and green house tests can also be involved (Mauria, 2000) and the new (candidate) varieties are compared with existing varieties that are kept as reference collection. The present investigation comprised of 12 varieties of fodder sorghum (Sorghum bicolor (L.) Moench) was undertaken for genetic variability among varieties of sorghum based on protein profile of seed storage protein. The banding pattern of total soluble protein showed considerable variation among the twelve fodder sorghum varieties. Seven protein marker bands were present in all varieties but the intensity and thickness varies from variety to variety. The analysis of total soluble protein banding pattern in all the fodder sorghum varieties were carried out along with the standard protein molecular weight marker having 14.4 to 116.0 kDa. Unweighted paired group method with arithmetic mean (UPGMA) was used to construct dendrogram for all the twelve sorghum varieties. The dendrogram showed two major clusters at coefficient of 0.7774.
Pages: 08-11 | 942 Views 37 Downloads
How to cite this article:
DG Gend, VB Jadhav, SN Kolekar. Genetic variability among variety of sorghum [Sorghum bicolor (L.) Moench] based on protein profile of seed storage proteins. Int J Chem Stud 2017;5(6):08-11.