International Journal of Chemical Studies
Vol. 5, Issue 5 (2017)
Detection of brucella organism in crossbred cattle based on serological and molecular assay
Author(s): Koushik Kakoty, Karam Amarjit, Manoj Kumar Kalita, Maradona Nath and Raju Kumar Dewry
Abstract: A total of 110 sera sample and whole blood samples were collected separately from unorganized dairy herds of Jersey and Holstein-Friesian (HF) crossbred cattle to evaluate the sero-prevalence of brucellosis by Rose Bengal Plate Test (RBPT), indirect ELISA and its PCR assay. The prevalence rate was recorded 13.63% by RBPT and 20.90% by ELISA respectively. Milk samples (82) were screened by Milk Ring Test (MRT) with a sero-positivity of 17.07%. The disease predisposition with respect to breeds revealed that the prevalence was significantly higher in Holstein Friesian (23.07%) than Jersey (17.77%) crossbred. According to age group the prevalence of brucellosis was higher in animals of age >4years (27.27%) followed by animals in the age groups of 2–4 years (22.05%) and least in animals of 1-<2 years of age (10%). With regard to history of reproductive failure, the highest prevalence of Brucellosis was found among the animals having previous history of abortion (61.90%) followed by retention of placenta (44.44%) and repeat breeding (18%). Screening of whole blood sample and milk sample by genus specific (bcsp-31 gene) as well as species specific IS711 gene based PCR revealed that 21(19.09%) blood sample and 9 (10.97%) milk samples were found positive.
Pages: 264-266 | 603 Views 9 Downloads
How to cite this article:
Koushik Kakoty, Karam Amarjit, Manoj Kumar Kalita, Maradona Nath, Raju Kumar Dewry. Detection of brucella organism in crossbred cattle based on serological and molecular assay. Int J Chem Stud 2017;5(5):264-266.