International Journal of Chemical Studies
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P-ISSN: 2349-8528, E-ISSN: 2321-4902   |   Impact Factor: GIF: 0.565 NAAS Rating: 5.31 NEW

International Journal of Chemical Studies

Vol. 5, Issue 5 (2017)
Effect of antioxidant on in vitro maturation of bovine follicular oocytes


Author(s): A Das, PM Barua, P Borah, BC Deka, J Sonowal and MC Borah

Abstract: The present study was conducted to assess the effect of α-tocopherol on in-vitro maturation of bovine follicular oocytes. The aspirated follicular oocytes were in-vitro cultured in TCM-199 medium at 38.5ºC with 5% CO2 for 24 hours in a CO2 incubator. The medium TCM-199 supplemented with follicular fluid, oestradiol-17β, p-FSH, gentamicin, sodium pyruvate, cysteamine and fetal bovine serum was used as control. In the treatment groups, the control medium was supplemented with 100 µM and 150 µM α-tocopherol. The rates of cumulus cell expansion recorded in 100 µM, 150 µM α-tocopherol supplemented and the control group of oocytes were 74.28 ± 1.66, 73.59 ± 0.78 and 74.46 ± 2.68 per cent and polar body formation were 50.06 ± 2.40, 45.43 ± 4.20 and 44.02 ± 4.37 per cent, respectively.

Pages: 2432-2434  |  432 Views  17 Downloads

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How to cite this article:
A Das, PM Barua, P Borah, BC Deka, J Sonowal, MC Borah. Effect of antioxidant on in vitro maturation of bovine follicular oocytes. Int J Chem Stud 2017;5(5):2432-2434.
International Journal of Chemical Studies