Vol. 5, Issue 5 (2017)
Detection of pesticide residues in vegetables by using rapid test KIT
Author(s): S Easwari and R Jayakumar
Abstract: Rapid test kit is a simple and inexpensive tool when compared to conventional analysis of pesticide residues through gas chromatography and high performance liquid chromatogaphy. In the present investigation the organophosphorus insecticides viz., chlorpyrifos, dichlorvos, dimethoate, malathion, methyl parathion, monocrotophos, phosphamidon, profenofos, quinalphos and triazophos insecticides were assessed with different chromogenic reagents. Initial screening of the chromogenic reagents to detect organophosphorus insecticides was done through paper strip method. In paper strip method, development of brown colour was observed for dimethoate using iodine and silver nitrate as chromogenic reagents. Phosphamidon and quinalphos were detected by developing brown and white colour using silver nitrate and sodium hydroxide. The darkness of colour on the spot was increased with increased concentrations. The fortification studies were conducted to determine the dimethoate, methyl parathion, phosphamidon and quinalphos through paper strip method. The results reported that using iodine and silver nitrate brown colour was appeared in dimethoate residual extract. Where as in phosphamidon and quinalphos, the residual extract when treated with silver nitrate the colour obtained was not comparable with standards. Validation was done for dimethoate using standards and residual extracts and the results were comparable with standards as well fortified residual extracts. From the above experimental results, a rapid test kit was developed for dimethoate residues detection. The developed rapid test kit was used to determine the pesticide residues present in market vegetable samples.
Pages: 2274-2277 | 1481 Views 207 Downloads
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How to cite this article:
S Easwari, R Jayakumar. Detection of pesticide residues in vegetables by using rapid test KIT. Int J Chem Stud 2017;5(5):2274-2277.