Vol. 5, Issue 5 (2017)
Chromatin Immunoprecipitation (ChIP) Assay - A Tool for Functional Analysis of Gene Expression
Author(s): Sidharth Prasad Mishra, Chinmoy Mishra, Siddhant Sekhar Sahoo, Kamdev Sethy and Kumaresh Behera
Abstract:
The Whole Genome Sequencing Project developed enormous amount of data about the genetic architecture of organisms. But mere structural characterization of genome will ultimately not be the concluding stage; a functional characterization is essentially required for the complete understanding of genome biology. The temporal and spatial regulation of gene expression though occurs at several levels of central dogma process but regulation at transcriptional level is most important in vertebrates. Identification of transcription factors controlling gene expressions is becoming utmost important for understanding the regulation of gene expression. The Chromatin Immunoprecipiation (ChIP) assay provides a powerful technique for identifying the in vivo association of transcription factors with regulatory elements of a gene. ChIP allows enrichment of genomic regions which are associated with specific transcription factors, histone modifications, and indeed any other epitopes which are present on chromatin. The original ChIP methods used site-specific PCR and Southern blotting to confirm which regions of the genome were enriched, on a candidate basis. The combination of ChIP with genomic tiling arrays (ChIP-chip) and next generation sequencing now allowed a more unbiased approach to map ChIP-enriched sites.
Pages: 2039-2044 | 668 Views 50 Downloads
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How to cite this article:
Sidharth Prasad Mishra, Chinmoy Mishra, Siddhant Sekhar Sahoo, Kamdev Sethy, Kumaresh Behera. Chromatin Immunoprecipitation (ChIP) Assay - A Tool for Functional Analysis of Gene Expression. Int J Chem Stud 2017;5(5):2039-2044.
