Vol. 5, Issue 5 (2017)
Efficient elicitor and precursor induced resveratrol production in suspension cultures of Arachies hypogea
Author(s): Raghavendra S, Satish D, Dileep Kumar Masuthi, Padmanabha K, Biradar IB and Hadimani HP
Abstract: Plant secondary compounds eliciting pharmacological or toxicological effects have long been processed for varied objective and they symbolize the perpetual compassion of nature by every means and are expressed in diverse human traditions. Plant cell cultures bequeath an alternative means for producing secondary metabolites. The current research is an effort to study the influence of elicitor salicylic acid and precursor phenylalanine and malonyl co A on the production of resveratrol, the most highly active phytochemical present in A. hypogea. Suspension cultures were established on Murashige and Skoog (MS) medium supplemented with 2, 4-dichlorophenoxyacetic acid (2, 4-D- 13.57 μM) and 6-benzylaminopurine (BAP- 0.88μM). Elicitor and precursor at different concentrations were supplemented to the medium and resveratrol produced was quantified by high performance liquid chromatography (HPLC). Several-fold increase in resveratrol concentration compared to the control was observed in elicited cultures. Precursor feeding (127.86±7.62) was found to be superior that elicited higher concentration of resveratrol, while noteworthy elicitation compared to precursor was observed in salicylic acid (74.45±5.62) on resveratrol production in the cell suspension culture of A. hypogea. Loss of cell viability was observed at high concentrations in both elicitor-precursor treated cultures. The results imply the production of resveratrol in elicitor-precursor treated suspension cultures are several times higher than in peanut plant and conceivably provide a new outlook for the further development.
Pages: 621-626 | 988 Views 55 Downloads
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How to cite this article:
Raghavendra S, Satish D, Dileep Kumar Masuthi, Padmanabha K, Biradar IB, Hadimani HP. Efficient elicitor and precursor induced resveratrol production in suspension cultures of Arachies hypogea. Int J Chem Stud 2017;5(5):621-626.
