Vol. 5, Issue 4 (2017)
In vitro biosynthesis and characterization of biosilver nanoparticles of Pseudomonas putida LUA 15.1 and their potential as antibacterial agents
Author(s): Amna Devi, Ankita Sharma, Himani Sharma, Shashikant Sharma, Pankaj Raghuvanshi and Poonam Shirkot
Biosilver nanoparticles are playing an important role in various field such as in agriculture, cosmetics, bioremediation and biomedical. In present investigation, in vitro synthesis of silver nanoparticles using Pseudomonas putida LUA 15.1 was carried out after optimization of various parameters. Optimum condition parameters for maximum synthesis of silver nanoparticles was selected which included TY broth containing 5g/l tryptone, 3g/l yeast extract, 1.5 mM AgNO3, whereas CuSO4 led to decline in silver nanoparticles synthesis. TY broth (pH; 8.0) using optimum condition was used for in vitro synthesis of silver nanoparticles at 35°C for 24 hrs of incubation. Nanoparticles synthesis indicated by change in colour from pale yellow to brown red was confirmed by UV–Vis spectroscopy with absorbance peak obtained between 330-460 nm. Characterization of biosilver nanoparticles was carried using SEM, DLS and FTIR. These biosilver nanoparticles found to be monodispersed, spherical in shape with size range of 10-25 nm and FTIR spectrum revealed presence of alkenes, phenylester and 1,2,4-trisubstituted biomolecules and these were tested for antibacterial activity against four bacterial plant using agar well diffusion test producing significant zones of inhibition against bacterial pathogens after 24 hrs of incubation.
Pages: 1817-1824 | 721 Views 13 Downloads
How to cite this article:
Amna Devi, Ankita Sharma, Himani Sharma, Shashikant Sharma, Pankaj Raghuvanshi, Poonam Shirkot. In vitro biosynthesis and characterization of biosilver nanoparticles of Pseudomonas putida LUA 15.1 and their potential as antibacterial agents. Int J Chem Stud 2017;5(4):1817-1824.