International Journal of Chemical Studies
Vol. 5, Issue 4 (2017)
Effect of surface sterilant for reducing microbial contamination of field grown strawberry explants intended for in vitro culture
Author(s): uvalaxmi Palei, AK Das, DK Dash and GR Rout
Abstract: An effective disinfection method for strawberry (Fragaria x ananassa Duch.) cv. Chandler micro propagation using runner tips and nodal segments as explants was developed. The explants were surface sterilized with different sterilants for different durations. The present studies on the effect of different regimes of sterilization revealed that minimum contamination percentage, maximum survival percentage, minimum nonresponsive explants and highest shoot length were obtained from explants runner tips and nodal segments when treated with 0.1% mercuric chloride for 7 min and 0.1% mercuric chloride for 7 min plus ethyl alcohol 70% for 30 s respectively. It was found that a single sterilant is more effective than the combination in case of runner tip but combination of mercuric choride and ethyl alcohol was found to be best when the explants nodal segment was used for in vitro culture.
Pages: 1476-1479 | 460 Views 18 Downloads
How to cite this article:
uvalaxmi Palei, AK Das, DK Dash, GR Rout. Effect of surface sterilant for reducing microbial contamination of field grown strawberry explants intended for in vitro culture. Int J Chem Stud 2017;5(4):1476-1479.