International Journal of Chemical Studies
Vol. 5, Issue 4 (2017)
Effect of oocyte retrieval techniques on yield and quality of buffalo (Bubalus bubalus) oocytes
Author(s): Dr. Arul V
Abstract: The aim of this study was to investigate the recovery rate and quality of oocytes by slicing and aspiration methods. The cumulus oocytes complexes and denuded oocytes were collected from 2 ̶ 8 mm follicles from local abattoir’s ovaries. Although, the slicing method yielded a significantly (P<0.0001) large number (3.75 oocytes per ovary) of oocytes than the aspiration method (2.65 oocytes ovary). However, the slicing method resulted in providing large quantity of good quality oocytes compared to the aspiration techniques. Therefore it was concluded that slicing the ovarian surface is a better method to recover more number of good quality oocytes from ovaries for in vitro studies.
Pages: 1221-1225 | 551 Views 9 Downloads
How to cite this article:
Dr. Arul V. Effect of oocyte retrieval techniques on yield and quality of buffalo (Bubalus bubalus) oocytes. Int J Chem Stud 2017;5(4):1221-1225.