International Journal of Chemical Studies
Vol. 5, Issue 4 (2017)
Effect of melatonin on in vitro maturation of buffalo (Bubalus bubalis) oocytes
Author(s): Dr. V Arul, Dr. VS Gomathy and V Brindha
Abstract: Objective: Nutrients and antioxidants in the medium of immature oocyte have a profound effect on maturation, fertilization and development of resulting embryos. In this study the effects of varying dose of melatonin as an antioxidant agent on maturation capacity were evaluated in immature oocytes of buffaloes. Materials and Methods: In this experimental study, immature oocytes were harvested from ovaries of buffaloes slaughtered at abattoirs of Chennai Corporation. Oocytes were harvested from collected ovaries by using different techniques and within 30 minutes it was transferred to in vitro maturation medium containing varying doses of melatonin (10-4, 10-6, 10-8, 10-10, 10-12) and without melatonin (Control) for 22-24 hours. Maturation was monitored using an inverted microscope. Results: Melatonin in the concentration of 10-6 M had the most effect on maturation of oocytes in in vitro maturation medium (p<0.05) when compared to the control. This study concludes that melatonin at the rate of 10-6 M in the serum free IVM plays an effective role in attaining the maximal maturation rate of buffalo oocytes.
Pages: 1133-1140 | 382 Views 6 Downloads
How to cite this article:
Dr. V Arul, Dr. VS Gomathy, V Brindha. Effect of melatonin on in vitro maturation of buffalo (Bubalus bubalis) oocytes. Int J Chem Stud 2017;5(4):1133-1140.