International Journal of Chemical Studies
Vol. 5, Issue 4 (2017)
Phenotypic and molecular screening for diseases resistance of apple cultivars and selections against apple scab (Venturia inaequalis)
Author(s): Muneer Ahmad Sheikh, KM Bhat, JI Mir, MA Mir, Sajad Un Nabi, MA Bhat, Hilal Ahmad, Wajida Shafi, Shafia Zaffer, Sumaira Jan and Waseem H Raja
Abstract: Apple scab is one of the most widespread diseases of apple trees causing huge economic losses both quantitatively and qualitatively. The increased expenses associated with labor and fungicides justify the investigation of an alternative, effective, least expensive resistance approach to deal with this disease. In present study we evaluated different cultivars and selections against apple scab disease, using phenotypic and molecular approaches. Phenotypic screening using leaf detach method showed that, in certain cultivars and selections fungus did not proliferate macroscopically and the leaves remained alive, green and also showed hypersensitive response, which gives indication of resistance against apple scab. To validate the results of phenotypic screening, all twenty four cultivars were evaluated for the presence of Vf gene conferring resistance against apple scab disease using gene specific primers. Certain gene specific markers amplified only one fragment in resistant cultivars and hence confirmed the presence of Vf gene in these cultivars. Some amplified fragments in both susceptible and resistant lines, hence couldn’t distinguish between resistant and susceptible ones. Present study confirmed the resistance both by phenotypic and molecular screening, hence can play an important role in designing future molecular breeding programme in apple for introgression of scab resistant genes.
Pages: 1107-1113 | 630 Views 33 Downloads
How to cite this article:
Muneer Ahmad Sheikh, KM Bhat, JI Mir, MA Mir, Sajad Un Nabi, MA Bhat, Hilal Ahmad, Wajida Shafi, Shafia Zaffer, Sumaira Jan, Waseem H Raja. Phenotypic and molecular screening for diseases resistance of apple cultivars and selections against apple scab (Venturia inaequalis). Int J Chem Stud 2017;5(4):1107-1113.