International Journal of Chemical Studies
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P-ISSN: 2349-8528, E-ISSN: 2321-4902   |   Impact Factor: GIF: 0.565 NAAS Rating: 5.31 NEW

International Journal of Chemical Studies

Vol. 5, Issue 4 (2017)
Isolation and identification of effective Rhizobium germplasm for increasing productivity of Kulthi (Dolichos biflorus L.) under climatic conditions of Chhattisgarh


Author(s): Singh RN, Singh AK, Gupta SB, Sengar SS, Kumar Rahul

Abstract: A field experiments were conducted at Sarguja, Bastar and Raipur C.G. continuous three year to select location specific Rhizobium isolates(s) for Horse gram local name Kulthi (Halba) through systematic screening of local Rhizobium germplasm comparing with local checks. In addition to newly Rhizobium germplasm of 313 new Rhizobium isolates(s) were isolated from soils of Sarguja, Korba and Bastar districts under the present investigation. On the basis of Biological Nitrogen Fixing performance 10 best isolates were selected through screening of the 313 newly collected germplasm under glass house conditions. Above 10 best local isolates and local check as native Rhizobia with General Recommended Dose of fertilizers were considered for studies in field conditions. Study under glass house sand culture devoid of nitrogen source conditions clearly revealed the variation in the symbiotic effectiveness of the tested rhizobial isolates. The nitrogen fixing efficiency of national and local isolates were studied in terms of parameters like plant height, nodulation, biomass accumulation, plant nitrogen content and its uptake by Horse gram var. AK-21. The keeping in view, seed yield of Horse gram was recorded 486.5, 467.04, 462.59, 453.14, 444.80, 442.02, 436.46, 425.34 425.34 and 414.22 kg/ha due to inoculation with local isolates numbered, 11, 56, 41, 2, 51, 12, 67, 47, 59, and 10 respectively. The seed yield 139.00 kg/ha was recorded the lowest and 486.5 kg/ha was observed the highest among all isolates under the field study in absolute control and isolate no 11, respectively.

Pages: 1069-1073  |  447 Views  14 Downloads

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How to cite this article:
Singh RN, Singh AK, Gupta SB, Sengar SS, Kumar Rahul. Isolation and identification of effective Rhizobium germplasm for increasing productivity of Kulthi (Dolichos biflorus L.) under climatic conditions of Chhattisgarh. Int J Chem Stud 2017;5(4):1069-1073.
International Journal of Chemical Studies