International Journal of Chemical Studies
Vol. 5, Issue 3 (2017)
Genetic diversity analysis of common carp (Cyprinus carpio var. Communis) and Labeo rohita (hamilton, 1822) collected from hatchery by using microsatellite markers
Author(s): Mohd Danish and IJ Singh
Abstract: The present study deals with genetic diversity analysis of Cyprinus carpio var. communis and Labeo rohita (Hamilton, 1822) collected from hatchery stock through microsatellite marker. Total 20 microsatellite primers were designed by using software Primer-BLAST and Primer-3. A total of 12 microsatellite loci were successfully amplified. After performing native PAGE using amplified 50 DNA samples each, POP GENE Version 1.32 was used to calculate microsatellite variation. The average expected Nei’s genetic diversity ranged from 0.328 to 0.529 with mean value of 0.458 for Labeo rohita across all loci from hatchery whereas the average expected gene diversity ranged from 0.392 to 0.537 with mean value of 0.483 for Cyprinus carpio var. communis across all loci from hatchery. The observed and expected heterozygosity ranged from 0.2237 to 0.3326 and 0.2786 to 0.3763 respectively for Labeo rohita from hatchery. The mean value of observed heterozygosity was 0.2864 and that of expected heterozygosity was 0.3238. Mean Fis value were found to be 0.193 at all loci in hatchery whereas for common carp the observed and expected heterozygosity ranged from 0.2659 to 0.3910 and 0.3145 to 0.4129. The mean value of observed heterozygosity was 0.3350 and that of expected heterozygosity was 0.3731. Mean Fis values for common carp were found to be 0.184 at all loci in hatchery. Mean values for Shannon’s information index for all microsatellite loci were 1.1091 for rohu and 1.1320 for Cyprinus carpio from hatchery stock. Slightly more level of observed heterozygosity in Cyprinus carpio var. communis than Labeo rohita from hatchery might be due to presence of more differentiated stocks. Lesser value of observed heterozygosity in Labeo rohita from hatchery than Cyprinus carpio var. communis might be possibly due increase in incidents of inbreeding in successive generations owing to lack of regular germplasm exchange of appropriate genetic diversity. The microsatellite analysis showed that Cyprinus carpio var. communis of hatchery is more genetically diversed and genetically differentiated as compared to Labeo rohita.
Pages: 513-518 | 550 Views 10 Downloads
How to cite this article:
Mohd Danish, IJ Singh. Genetic diversity analysis of common carp (Cyprinus carpio var. Communis) and Labeo rohita (hamilton, 1822) collected from hatchery by using microsatellite markers. Int J Chem Stud 2017;5(3):513-518.