International Journal of Chemical Studies
Vol. 5, Issue 3 (2017)
Formulation of liquid spawn base of paddy straw mushroom, Volvariella volvacea (Bull. Ex Fr.) sing
Author(s): Nannapaneni Kiran Kumar, Akkanna Subbiah Krishnamoorthy and Ayyanar Kamalakannan
Abstract: Mushroom spawn in liquid form could be more handily employed and its dispersion would be greatly superior to solid spawn. Underpinning this, an isotonic liquid spawn base is formulated with gum acacia, trehalose, carboxymethyl cellulose (CMC), glycerol, polyethylene glycol (PEG) and aloe gel, based on their water activity for uniform suspension and preservation of chlamydospores and basidiospores. The spores were suspended in the formulation and viability counts were recorded at 15 days interval. The outcome perspicuously indicated that the chlamydospores suspended in PEG were found to be the best even after 45 days (1.5×104 spores ml-1) followed by aloe gel based formulation (1.2×104 spores ml-1). In the case of basidiospores, the isotonic formulation with glycerol as preservative unveiled more number of viable spore counts (8.7×104 spores ml-1) followed by aloe gel (7.5×104 spores ml-1). The efficiency of liquid formulation tested in terms of laccase activity indicated that the substrate seeded with chlamydospore based formulation had exhibited comparatively more laccase activity than that of the basidiospore based formulation.
Pages: 138-142 | 801 Views 21 Downloads
How to cite this article:
Nannapaneni Kiran Kumar, Akkanna Subbiah Krishnamoorthy, Ayyanar Kamalakannan. Formulation of liquid spawn base of paddy straw mushroom, Volvariella volvacea (Bull. Ex Fr.) sing. Int J Chem Stud 2017;5(3):138-142.