Vol. 5, Issue 2 (2017)
Effect of melatonin on the expression profile of HSP60 and HSP70 in Beetal and Assam Hill Goat exposed to direct sunshine during summer in Assam
Author(s): Dilruba Hasin, Arundhati Bora, Jiten Goswami, Isfaqul Hussain, Abdul Saleque, Probadh Borah, Iftikar Hussain, Anubha Barua, Biren Kumar Sarmah, Ranjit K Bora and Mithu Dutta
Abstract: The present investigation was carried out to evaluate the effect of melatonin on mRNA expression of HSP60 and HSP70 genes in the peripheral blood mononuclear cells (PBMC) in Beetal goats in Assam with reference to native breed of goat i.e., Assam Hill Goat (AHG) that were exposed to direct sunshine in summer. The study included 18 AHG and 18 Beetal goats of uniform age group (5-6 months), which were subdivided into three groups containing 6 animals in each group. One group was kept indoor while other two groups of each breed were exposed to direct sunshine @ 6 hours/day for a period of 30 days. Melatonin was fed @ 6 mg/goat to one of the two sunshine exposed groups. Blood samples were collected from all the animals before and after the exposure at weekly interval and real-time polymerase chain reaction was applied to investigate mRNA expression of HSP60 and HSP70 genes. The relative expression of HSP60 and HSP70 mRNA was found to be significantly higher (P <.0.01) in the sunshine exposed groups of Beetal goats in comparison to AHG. In the melatonin fed group, the expression was significantly higher than the group without melatonin of either Beetal or AHG. The relative HSP60 and HSP70 mRNA expression in both the sunshine exposed groups of either Beetal or AHG increased significantly from day one to day 21 and then decreased at 28 days. The expressions were significantly higher in the melatonin fed group in comparison to the group without melatonin in different days after day one.
Pages: 268-273 | 1221 Views 85 Downloads
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How to cite this article:
Dilruba Hasin, Arundhati Bora, Jiten Goswami, Isfaqul Hussain, Abdul Saleque, Probadh Borah, Iftikar Hussain, Anubha Barua, Biren Kumar Sarmah, Ranjit K Bora, Mithu Dutta. Effect of melatonin on the expression profile of HSP60 and HSP70 in Beetal and Assam Hill Goat exposed to direct sunshine during summer in Assam. Int J Chem Stud 2017;5(2):268-273.
