Vol. 5, Issue 2 (2017)
Induction of lignolytic enzyme activities in different agro residues by the white rot fungi, Pleurotus sajar-caju
Author(s): Thiribhuvanamala G, Kalaiselvi G, Parthasarathy S and Anusha B
Abstract: The white rot fungi, Pleurotus sajar-caju secretes the major lignin modifying enzymes Laccase (Lac) and Manganese peroxidase (MnP) with low levels of Lignin peroxidase (LiP) during their secondary metabolism that is capable of mineralizing lignin. Assay of enzymes under liquid screening test indicated significant levels of laccase (0.56 U/ml), LiP (0.054U/ml) and MnP (0.21 U/ml) by P. sajar-caju. Induction of lignolytic enzymes by P. sajar-caju was tested in different farm agro wastes viz., sugarcane, banana, millets, pulses, cocopeat, cocoa wastes, coir pith and oil palm under solid state fermentation. Among the enzymes, laccase and MnP was produced in significant levels by P. sajar-caju from 21st to 28th day under solid state fermentation (SSF). Among the substrates tested, significant levels of laccase was secreted in residues of oil palm, banana, followed by sugarcane and pulses where as maximum MnP was produced residues of oil palm, banana, sugarcane, cotton and pulses. LiP though produced was in trace amounts which showed preference over the substrates like sugarcane banana, pulses and oilseeds. Substitution of malt extract medium with organic substrates viz., wheat bran and ground nut cake each @ 5 per cent and inorganic substrates viz., copper sulphate and manganese sulphate each at 150 µM enhanced the lignolytic enzymes of P. sajar-caju in liquid medium. The results from this study offers scope for application of white rot fungi P. sajar-caju for biodegradation of agrowastes as well for recycling of the agro wastes for the commercial production of edible mushrooms under Integrated farming system to benefit the farming community as well to tap industrially important lignolytic enzymes like laccases.
Pages: 89-94 | 1452 Views 54 Downloads
How to cite this article:
Thiribhuvanamala G, Kalaiselvi G, Parthasarathy S, Anusha B. Induction of lignolytic enzyme activities in different agro residues by the white rot fungi, Pleurotus sajar-caju. Int J Chem Stud 2017;5(2):89-94.