International Journal of Chemical Studies
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P-ISSN: 2349-8528, E-ISSN: 2321-4902   |   Impact Factor: GIF: 0.565 NAAS Rating: 5.31 NEW

International Journal of Chemical Studies

Vol. 5, Issue 2 (2017)
Optimization of carotenoid pigment production by yeast Sporobolomyces sp. using response surface methodology


Author(s): M RA Manimala and R Murugesan

Abstract: The Response Surface Methodology (RSM) technique was performed to increase the carotenoid pigment production of Sporobolomyces sp. The Central composite design involving the variables pH (A), fermentation time (B) and incubation temperature(C) for the production of carotenoid pigment by Sporobolomyces sp was standardized. Data analyzed using a second order polynomial equation resulted in the optimized process condition for pigment production was 120 h growth at 25 °C in a Yeast extract dextrose broth of pH 5.0. Using this experimental design, the total carotenoid production yield increased from 674.34 to 990.87 µg g-1. The model showed that the value of R2 0.9986 was high and p- value of interaction of variance was <0.0001. Hence the model can said to be significant.

Pages: 77-80  |  873 Views  14 Downloads

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How to cite this article:
M RA Manimala, R Murugesan. Optimization of carotenoid pigment production by yeast Sporobolomyces sp. using response surface methodology. Int J Chem Stud 2017;5(2):77-80.
International Journal of Chemical Studies