Vol. 4, Issue 3 (2016)

Method: A reverse phase HPLC method was developed using Bondapak C18 10 µm (3.9 x 300 mm) Capillary column was employed for investigation and mobile phase of composition Acetonitrile: Water in the ratio of 50:50 v/v at a flow rate of 1.0 mL/min with UV detection at 240 nm for Meprobamate.

Results: The retention time of the drug was 7.0 minutes. The developed method was validated for specificity, linearity, precision, accuracy and robustness as per ICH guidelines. Linearity was found in the range of 0.0375-0.225mg/ml. The mean recovery of the drug was 99.6%. The proposed method could be used for routine analysis of Meprobamate in their dosage forms.

Conclusion: The proposed method is accurate, precise, simple, sensitive and rapid and can be applied successfully for the estimation of Meprobamate in bulk and in pharmaceutical formulations without interference and with good sensitivity.