International Journal of Chemical Studies
Vol. 4, Issue 3 (2016)
Evaluation of cytotoxic activity of various extracts of sweet cherry (Prunus avium) against human colorectal adenocarcinoma HT-29 cell line
Author(s): Ch. Manogna, Asish Bhaumik, T. Haritha, SK. Nasreen, M. Sucharitha, M. Uttara
Abstract: HT-29 is a humanrncolorectal adenocarcinoma cell line with epithelial morphology. These cells arernsensitive to the chemotherapeutic drugs 5-fluorouracil and oxaliplatin, whichrnare standard treatment options for colorectal cancer. In addition to being a xenograftrntumor model for colorectal cancer, the HT-29 cell line is also used as an in-vitrornmodel to study absorption, transport, and secretion by intestinal cells. Underrnstandard culture conditions, these cells grow as a nonpolarized,rnundifferentiated multilayer. Altering culture conditions or treating the cellsrnwith various inducers, however, results in a differentiated and polarizedrnmorphology, characterized by the redistribution of membrane antigens andrndevelopment of an apical brush-border membrane. The main objective of thernpresent research work is to isolate the bioactive molecules and evaluate the inrnvitro cytotoxic activity of methanolic and chloroform extracts of sweetrncherry (MEC and CEC) of Prunus avium. The in vitro cytotoxicrnactivity was carried out against human colorectal adenocarcinoma cell linernHT-29 by SRB assay. The results obtained from the in-vitro studiesrnperformed by SRB assay by using human colorectal adenocarcinoma cell line HT 29rndisplayed that the various extracts of sweet cherry (MEC and CEC) possessed arnvery good anticancer activity. From the present studied it had been concludedrnthat MEC and CEC, all were exhibiting the potential capability to kill therncancer cell when compared with standard drug 5-FU and the cell growth inhibitionrnof MEC and CEC was found to be the highest 93.91% at 10 µg (IC50 =rn2.5 µg/ml) and 94.25% % at 10 µg (IC50 = 2.1 µg/ml
Pages: 17-21 | 1380 Views 33 Downloads
How to cite this article:
Ch. Manogna, Asish Bhaumik, T. Haritha, SK. Nasreen, M. Sucharitha, M. Uttara. Evaluation of cytotoxic activity of various extracts of sweet cherry (Prunus avium) against human colorectal adenocarcinoma HT-29 cell line. Int J Chem Stud 2016;4(3):17-21.