International Journal of Chemical Studies
Vol. 4, Issue 1 (2016)
Qualitative investigation of phytochemicals and brine shrimp lethality test of the root, stem bark and leaves extract of Isoberlinia doka (Fabaceae)
Author(s): Abdu Z, Dimas K, Sunday AO, Isyaka MS, Sa’id J
Abstract: Isoberlinia doka was selected in this study on the bases of its use in folk medicine by Lala tribe of Adamawa State - Nigeria to cure Jaundice and Headache diseases. Extracts prepared by soxhlet extraction with hot methanol from the plant were macerated and screened for the presence of secondary metabolites and activity in the brine shrimp (Artemia salina Leach) lethality test (BST). In the phytochemical screening, n-hexane extracts of the leaves, stem bark and root showed the presence of few secondary metabolites with the methanol extracts indicating the presence of most of the secondary metabolites (Tables 2-4). Extract of the root and stem bark exhibited high lethality on brine shrimp larvae with LC50 = 63.1μg/ml and 70.8μg/ml respectively. The leaves extracts exhibited a moderate potent activity at LC50 = 436.5 μg/ml. Maximum mortalities occurred at 1000μg/ml concentration while the least mortalities happened to be at 65.5μg/ml concentration. The mean results of mortality percentage of the brine shrimp versus the log of concentrations were plotted using the Microsoft Excel spreadsheet application, which also formulated the regression equations. These equations were later used to calculate LC50 values for the samples tested with consideration of value greater than 1000 µg/ml, suggesting that the extract is nontoxic.
Pages: 112-116 | 1228 Views 22 Downloads
How to cite this article:
Abdu Z, Dimas K, Sunday AO, Isyaka MS, Sa’id J. Qualitative investigation of phytochemicals and brine shrimp lethality test of the root, stem bark and leaves extract of Isoberlinia doka (Fabaceae). Int J Chem Stud 2016;4(1):112-116.