International Journal of Chemical Studies
Vol. 2, Issue 5 (2015)
Validated RP - HPLC method for the determination of mepyramine maleate in bulk and pharmaceutical formulation
Author(s): M.Madhu, V.Sreeram, A.V.D. Nagendrakumar, Prof. T.V.Reddy
Abstract: A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Mepyramine Maleate in Bulk and Pharmaceutical Formulation. Isocratic elution at a flow rate of 1.0 ml/min was employed on symmetry µ Bondapak Phenyl 5 µm (3.9 mm x 30 mm) Column at ambient temperature. The mobile phase consisted of Buffer: Methanol: Acetonitrile in the ratio of 620:300: 250 v/v. The UV detection wavelength was 220 nm and 25 µl sample was injected. The retention time for Mepyramine Maleate was 9 min. The method gives linear response from 12.55 – 0.0625 mg/swab of Mepyramine Maleate. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Darunavir in the rapid and reliable determination of Mepyramine Maleate Pharmaceutical formulation.
Pages: 22-26 | 1244 Views 12 Downloads
How to cite this article:
M.Madhu, V.Sreeram, A.V.D. Nagendrakumar, Prof. T.V.Reddy. Validated RP - HPLC method for the determination of mepyramine maleate in bulk and pharmaceutical formulation. Int J Chem Stud 2015;2(5):22-26.