International Journal of Chemical Studies
Vol. 2, Issue 1 (2014)
Comparative evaluation of anti-bacterial efficacy of two different chemical irrigation protocols against Enterococcus faecalis in an infected root canal model by using polymerase chain reaction- an in vitro dental study
Author(s): Gnana Seelan R, Arvind Kumar A, Jonathan R, Uma Maheshwari, Benin P, Prakash Athiban
Abstract: The success of endodontic treatment depends upon the eradication of microbes from the root-canal system and the prevention of reinfection. Optimal irrigation is based upon the combined use of 2 or more irrigating solutions, in a specific sequence in order to achieve the goals of safe and effective irrigation. Materials Andmethods; 20 extracted Human lower premolars stored in 0.5% sodium hypochlorite at 4 0C were taken. The teeth were instrumented with Protaper rotary system up to (F3) after which the root canals were sterilized by autoclaving for 20 min at 121 0C. After which Enterococcus Faecalis (MTCC 2129) was incubated into all the samples for three weeks to form biofilm. Root canals were then randomly divided into 2 groups. Group 1(N-10) used ProRinse Endodontic Irrigation Probes (Dentsply). Group 2(N-10) Canal Brush (Coltene Whaledent Germany) After 24hrs the dentin shavings were taken using sterile gates glidden size 3 and were stored in sterile aluminium foils. The dentine chips were kept in a glass bottle containing 2 ml phosphate buffered saline and they were transferred into agar plates and the microbial load was calculated. Semiquantitative densitometry analysis of ampliﬁed CDNA bands was used to analyze the antimicrobial activity. Results; Results of our study showed that maximum antimicrobial activity was achieved in group 2. The microbial load was only 8% after 24 hrs in group 2 while in group 1 the microbial load was 43% after 24 hrs.Conclusion; During cleaning and shaping procedure a combination of effective irrigation and micro brush activation will effectively remove debris from root canals, thereby it reduces the bacterial load PCR can be used as a valuable tool for microbial identification.
Fig. 1: PCR Analysis: Semiquantitative densitometry analysis of ampliﬁed CDNA bands showing difference between control and treated samples
Pages: 41-45 | 1034 Views 9 Downloads
How to cite this article:
Gnana Seelan R, Arvind Kumar A, Jonathan R, Uma Maheshwari, Benin P, Prakash Athiban. Comparative evaluation of anti-bacterial efficacy of two different chemical irrigation protocols against Enterococcus faecalis in an infected root canal model by using polymerase chain reaction- an in vitro dental study. Int J Chem Stud 2014;2(1):41-45.