International Journal of Chemical Studies
Vol. 1, Issue 2 (2013)
Two new Spectrophotometric Approaches to the Simultaneous Determination of Ezetimibe and Atorvastatin Calcium
Author(s): Yehia Z. Baghdady*, Medhat A. Al-Ghobashy, Abdel-Aziz E. Abdel-Aleem, Soheir A. Weshahy
Abstract: Two rapid, simple and sensitive spectrophotometric methods for the quantitative analysis of ezetimibe (EZB) and atorvastatin calcium (ATVC) in bulk powder and combined tablet form have been developed and validated. The first method is bivariate calibration and the second method is ratio subtraction in conjunction with second derivative (D2) spectrophotometry. These methods are tested by analyzing synthetic binary mixtures of the above drugs and they are applied to commercial pharmaceutical preparation of the subjected drugs. Standard deviation is < 2 in the assay of raw materials and tablets. Methods are validated as per ICH guidelines and statistical comparison of the suggested methods with the reported spectrophotometric one using F and t tests showed no significant difference regarding both accuracy and precision.
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Fig. 1: Zero Order Absorption Spectra of 20 µg/mL Ezetimibe and 20 µg/mL Atorvastatin Calcium using Methanol as a Blank
Fig. 2: Linearity of the Absorbance At 232.6 nm and 251.0 nm of the Zero Order Spectra to the Corresponding Ezetimibe Concentration (5 – 30 ug/mL)
Fig. 3: Linearity of the Absorbance At 232.6 nm and 251.0 nm of the Zero Order Spectra to the Corresponding atorvastatin calcium concentration (10 – 35 ug/mL)
Pages: 14-25 | 948 Views 8 Downloads
How to cite this article:
Yehia Z. Baghdady*, Medhat A. Al-Ghobashy, Abdel-Aziz E. Abdel-Aleem, Soheir A. Weshahy. Two new Spectrophotometric Approaches to the Simultaneous Determination of Ezetimibe and Atorvastatin Calcium. Int J Chem Stud 2013;1(2):14-25.