Volume 1, Issue 4
Structure-Activity Relationship of Antioxidative Property of Hesperidin
Author(s): Dr. Kiran Mishra
Abstract: Present study was designed to investigate the structure-activity relationship of antioxidative property of hesperidin via a simple free radical scavenging system including, reducing power, chelating activity on Fe2+, free radical-scavenging, total antioxidant, superoxide radical scavenging, hydrogen peroxide scavenging and hydroxyl radical scavenging activities in an attempt to understand its mechanism of antioxidant activity, which may pave the way for possible therapeutic applications. Different antioxidant tests were employed, namely, reducing power, chelating activity on Fe2+, free radical-scavenging, total antioxidant, superoxide radical scavenging, hydrogen peroxide scavenging and hydroxyl radical scavenging activities. In addition, the results were compared with natural and synthetic antioxidants, such as α- tocopherol, ascorbic acid, butylated hydroxytoluene(BHT), butylated hydroxyanisole (BHA) and trolox. Hesperidin exhibited a strong reducing power, chelating activity on Fe2+, free radical-scavenging, hydrogen peroxide scavenging and hydroxyl radical scavenging activities. Antioxidant activity of hesperidin increased with increased concentrations, in the range of 20-140 μg/ml. Hesperidin also exhibited a strong superoxide radical scavenging activity. Total antioxidant activity of hesperidin and both standards decreased in the order of hesperidin > α-tocopherol > trolox > BHA > BHT. This study showed that hesperidin exhibited antioxidant activity in all tests and could be considered as a source of natural antioxidants.
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