Vol. 7, Issue 4 (2019)
Effect of presoaking treatments on in vitro seed germination of papaya to facilitate axenic explant production
Author(s): Bowiya KS, K Soorianathasundaram, C Kavitha and K Hemaprabha
Abstract: Studies were taken up to standardize in vitro seed germination of papaya cultivars CO 2 and TNAU papaya CO 8. As compared to other surface sterilisation treatments, lowest contamination was recorded in seeds disinfected with 5% sodium hypochlorite for one minute and sown under in vitro supplemented with Murashige and Skoog culture medium. Sowing the seeds under in vitro with seed coat removal was found to be better for enhancing in vitro germination. The influence of pre soaking of seeds was also studied by subjecting the seeds to varied chemical treatments involving GA3, KNO3, thiourea and boric acid. Presoaking of seeds in 500 ppm GA3 for 12 hours had resulted in higher germination percentage, seedling height and seedling vigour index whereas early emergence of seedlings was influenced by 10% KNO3. The in vitro generated seedlings can serve as the source of axenic explants for plant regeneration and further biotechnological studies.
How to cite this article:
Bowiya KS, K Soorianathasundaram, C Kavitha and K Hemaprabha. Effect of presoaking treatments on in vitro seed germination of papaya to facilitate axenic explant production. 2019; 7(4): 50-55.