International Journal of Chemical Studies
  • Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal
P-ISSN: 2349-8528, E-ISSN: 2321-4902   |   Impact Factor: GIF: 0.565 NAAS Rating: 5.31 NEW

International Journal of Chemical Studies

Vol. 7, Issue 2 (2019)
Evaluation and method development of promising hepatoprotective and cytoprotective antioxidant (Silymarin) by chromatography


Author(s): KJ Balasubramani, Rajesh Kumari Narwal and Shweta Rajani

Abstract: In recent days, application of herbal drugs is increasing for curing many diseases all over the world. Silymarin, a herbal compound used worldwide from ancient times have shown protective effects in the fields of nepherotoxicity, hepatotoxicity, viral hepatitis, neurotoxicity, lung diseases, depression and cancer. Chromatographic methods play significant role in the drug discovery, development, formulations and quality control in pharmaceuticals industry Validation of an analytical procedure is used to demonstrate that it is suitable for its intended purpose. Hence in the proposed study, we established a new reliable gradient HPLC method for the determination of Silymarin from herbal medicine. The method was validated with respect to specificity, accuracy, precision, linearity and range, limit of detection, limit of quantification, and robustness. This also includes the details ofsample preparation and method of analysis.

Related Graphics: Click here for more related graphics

Structure of Silymarin
Fig. 1: Structure of Silymarin

Accuracy/Recovery of Silymarin
Fig. 2: Accuracy/Recovery of Silymarin

Precision of Silymarin at 150µg/ml
Fig. 3: Precision of Silymarin at 150µg/ml

Pages: 24-29  |  230 Views  20 Downloads

download (4250KB)
How to cite this article:
KJ Balasubramani, Rajesh Kumari Narwal and Shweta Rajani. Evaluation and method development of promising hepatoprotective and cytoprotective antioxidant (Silymarin) by chromatography. International Journal of Chemical Studies. 2019; 7(2): 24-29.
Call for book chapter
International Journal of Chemical Studies
Journals List Click Here Research Journals Research Journals