Vol. 6, Issue 3 (2018)
Determination of insecticide imidacloprid in soil by HPLC
Author(s): Manish Kumar Verma and Naveen Kumar
Abstract: Imidacloprid is a new generation insecticide and highly active to protect the various vegetable crops, by controlling mites and insect pest. Imidacloprid is a systemic insecticide that enters the target pest via direct contact or ingestion. It is applied to seeds, soil and crops for controlling insects. Imidacloprid, a neurotoxic insecticide, belongs to family of the neonicotinoids. The analytical method using quick, easy, and effective for determination of insecticides in soil was developed using High Performance Liquid Chromatography (HPLC). 86 samples were collected from different region of U. S. Nagar, Uttarakhand to determine imidacloprid level, and positive samples were detected. The insecticide was extracted from soil using mobile phase acetonitrile and water (90:10, v/v). The determination of the target compounds were achieved in 8.0 min at flow rate of 1.0 ml/minute using reverse phase UV-detector for imidacloprid. The method showed that the limits of quantification (LOQ) ranged from 0.17- 0.25μg in all positive samples. A linear calibration curve was obtained by plotting area against concentration with a correlation coefficient of 0.995, while average recoveries were greater than 81 to 87 % in different samples. Out of 86 soil samples analysed 12 samples (10%) had detectable amount of residue levels for imidacloprid and 3 samples had residue levels above the recommended Maximum Residue Limit (MRL).
Pages: 3667-3669 | 402 Views 84 Downloads
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How to cite this article:
Manish Kumar Verma, Naveen Kumar. Determination of insecticide imidacloprid in soil by HPLC. Int J Chem Stud 2018;6(3):3667-3669.
