Vol. 6, Issue 3 (2018)
Influence of date of sowing and chickpea varieties on occurrence of collar rot and variability among isolates of Sclerotium rolfsii
Author(s): Shyam Lata Pal, Ashok Krishna, RK Singh and Narendra Birla
Abstract: The influence of three dates of sowing employing three chickpea varieties was studied on occurrence of collar rot and yield and variability among isolates of S. rolfsii on the basis of morphological and physiological characters. Variety JG-62 sown on 15 October showed maximum disease incidence and minimum in variety JAKI-9218 when sown on 1 November. Three isolates were grown on 6 culture media (broth) to study their colony characterstics. The growth was maximum on Czapeck’s dox solution followed by Ashby’s mannitol solution, Joffee’s solution, Asthana and Hawkar’s solution, Potato dextrose broth and least on Richard’s solution.. Indore isolate exhibited the maximum growth while minimum growth was obseved in Sehore isolate. The temperature of 25 oC and 30 oC were found suitable for the growth of S. rolfsii and Khandwa isolate (39.0 mm) recorded the maximum growth. The pH of 8.5-9.5 was suitable for the growth of all the isolates and Khandwa isolate (76.53 mm) recorded the maximum growth. Among the nitrogen sources sodium nitrate was found as the best for the growth of S. rolfsii (62.83 mm) and the maximum growth was recorded in Khandwa isolate (65.25 mm). Among the carbon sources maximum growth of S. rolfsii was recorded on sucrose (63.83mm) and the Khandwaisolate (50.08 mm) recorded the maximum growth. The isolates produced a large number of sclerotia (>300 sclerotia/plate), while Sehoreisolate produced fewer (<300 sclerotia/plate). The color of sclerotia was generally dark to reddishbrown at maturity.
Pages: 240-243 | 849 Views 70 Downloads
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How to cite this article:
Shyam Lata Pal, Ashok Krishna, RK Singh, Narendra Birla. Influence of date of sowing and chickpea varieties on occurrence of collar rot and variability among isolates of Sclerotium rolfsii. Int J Chem Stud 2018;6(3):240-243.
