Vol. 6, Issue 3 (2018)
Effect of dietary supplementation of probiotic and BMD on the growth performance of broiler chickens challenged with Clostridium perfringens induced necrotic enteritis
Author(s): Rathnapraba S, Kanagaraju P and Vijayarani K
Abstract: A challenging study was carried out for the period of 35 d to investigate the bioefficacy of probiotic and bacitracin methylene disalicylate (BMD) on growth performance and intestinal health against C. perfringens induced necrotic enteritis (NE) in broilers. 500 day old broiler chicks (Cobb 400) were divided into five treatment groups, each with 5 replicates of 20 birds each using a completely randomized design. The treatments were an uninfected control (T1), an infected control (T2), an infected group supplemented with probiotic at 500 g/tone of feed (T3), containing 2×1010 cfu/kg, an infected group supplemented with BMD at 500 g/tone of feed (T4) and an infected group supplemented with probiotic + BMD each at 500 g/tone of feed (T5). Necrotic enteritis was induced in the broilers by oral inoculation of 30,000 sporulated Eimeria necatrix oocysts on day 14 followed by Clostridium perfringens (MTCC No. 450, MTCC, Chandigarh) inoculation 1.0 mL (108 cfu/mL) on day 19 to 21 in group T3, T4 and T5. The data were recorded and analyzed by using SPSS software. The result indicated that the infected group revealed ballooning of the intestine, thickened mucosa and turkey towel appearance on postmortem examination. Probiotic supplementation significantly (p<0.05) increased weight gain on par with uninfected control group. The feed intake was significantly (p<0.05) reduced in challenged group. Feed conversion ratio was significantly (p<0.05) superior in probiotic+BMD supplemented group and worst in an infected group. It was observed that probiotic either alone or in combination with BMD improved ileal villi length by 9.41% and 10.03% respectively when compared with infected group. Escherichia coli and Clostridium perfringens counts (log CFU/g digesta) in duodenal contents were significantly (p<0.01) reduced in probiotic (T3) and probiotic+BMD (T5) supplemented groups compared with other groups. From this study, we can conclude that the supplementation of either probiotic or BMD at 500g/tone of feed had positive effect on the growth performance of commercial broilers.