International Journal of Chemical Studies
Vol. 5, Issue 1 (2017)
Phytochemical screening and biological activities of leaf of Foeniculum vulgare (Ensilal)
Author(s): Abebie Beyazen, Engeda Dessalegn and Wondimagegn Mamo
Abstract: The Foeniculum vulgare, known as fennel, has a long history of herbal uses as both food and medicine. Phytochemical screening, the total phenolic contents (TPC), total flavonoid contents (TFC), in vitro antioxidant, and antimicrobial activities of chloroform, water, methanol, and aqueous: methanol (20:80, v/v) extracts of Foeniculum vulgare leaf were investigated. The antioxidant activity was measured using 1, 1 Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, reducing power and total antioxidant activity by phosphomolybdate methods. TPC and TFC were evaluated using Folin–Ciocalteu and AlCl3 methods respectively. Foeniculum vulgare extracts were investigated for in vitro antibacterial screening by disc diffusion method against Bacillus megaterium, Enterococcus gallinarum, Escherichia coli and Pseudomonas aeruginosa. Aqueous: methanol (20:80, v/v) extract contained the highest TPC [(24.3 ± 0.99) mg GAE/g], and TFC [(18.92 ± 0.34) μg CE/mg] and showed the highest DPPH radical scavenging activity (IC50 = 69.68 ± 2.28 μg/mL). The results also showed that the aqueous: methanol (20:80, v/v) extract had strongest reducing ability (0.82 ± 0.06 nm) as compared to other extracts. Chloroform extract exhibited the highest total antioxidant capacity (1.94 ± 0.76 mg AAE/g) as determined by the phosphomolybdenum method. Except for total antioxidant activity, all antioxidant activities were positively correlated TPC and TFC. Of the Foeniculum vulgare extracts, the highest inhibition zone was found in aqueous: methanol (20:80, v/v) extracts of the (8.74 ± 0.12 mm) against Bacillus megaterium. The study revealed that antioxidant and antimicrobial activities of the crude extract of Foeniculum vulgare extracted by different solvents indicating a high potential to be used as natural antioxidants in food preservation as well as for preventing oxidative stress mediated human disorders.
Pages: 18-25 | 1119 Views 86 Downloads
How to cite this article:
Abebie Beyazen, Engeda Dessalegn and Wondimagegn Mamo. Phytochemical screening and biological activities of leaf of Foeniculum vulgare (Ensilal). International Journal of Chemical Studies. 2017; 5(1): 18-25.